Our Pipeline
JCSG has developed and integrated technologies covering the entire gene-to-structure process, enabling high-throughput structural biology.
- STAGE 1
Target Selection
Bioinformatics-driven selection of protein families and biomedically relevant targets.
- STAGE 2
Cloning & Expression
Parallel cloning and small-scale expression screening to identify viable constructs.
- STAGE 3
Protein Production
Scalable purification of soluble, well-behaved protein samples.
- STAGE 4
Crystallization
High-throughput nanoliter crystallization screening with automated imaging.
- STAGE 5
Data Collection
Synchrotron X-ray diffraction at SSRL with remote and automated workflows.
- STAGE 6
Structure Determination
Phasing, model building, refinement, validation, and PDB deposition.
See JCSG tools that power this pipeline →
Tools Used Across the Pipeline
Explore the tools, databases, and search services developed by JCSG.
- Structure Gallery — Browse JCSG-determined protein structures.
- BLAST vs. JCSG Targets — Search JCSG targets by sequence similarity.
- HMM vs. PSI Families — Profile-based search against PSI families.
- XtalPred — Predict protein crystallization propensity.
- qFit — Multiconformer modeling from electron density.
- Pubserver — Structural genomics literature search.
- Ligand Server — Browse ligands in PSI structures.
- QC Server — Validate crystallographic model quality.